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Two separate experiments were conducted using peppers and wasabi rhizomes to test for avoidance of capsaicin and isothiocyanate, respectively.
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Transgenic strains were tested for avoidance of MIP-1α, SST-28 and neurotensin.
To test directly whether, for avoidance, the function of egl-10 is required in ASH, we introduced in egl-10 mutants the wild-type egl-10 cDNA and expressed it specifically in the ASH neurons under the sra-6 promoter (see paragraph above " egl-10 interacts with goa-1, egl-30 and eat-16").
Trained flies were then tested for conditioned avoidance responses.
Larvae were tested for odor avoidance at fifth instar in the Y maze as previously described; they were not tested as third instars so as to minimize exposure to EA without the unconditioned stimulus (electric shock), and due to logistical problems with the small size of the caterpillars in an apparatus developed for larger individuals.
Interestingly, rivastigmine restored learning abilities in BDL rats tested for active avoidance (P = 0.003; Fig. 9A).
At least three independent lines for each construct were tested for the avoidance phenotype (see Additional file 2).
We are also testing for dehydration avoidance traits in S. pennellii (see above), which are likewise predicted to exhibit trade-offs.
Repeated testing for inhibitory avoidance every 24 h after training resulted in significant retention of fear memory in the groups treated with CE, vitexin, isovitexin and 6-C-glycoside-diosmetin or both flavonoidic fractions.
Mice with ablated SF1+ neurons in VMHdm/c were used to test for intact predator avoidance (Blanchard et al., 2005).
We determined the putative differences in the passive avoidance test for the two experimental groups by measuring the time taken by a mouse to enter the dark compartment after door opening (Fig. 6).
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