The RNA samples were tested for absence of inhibitors using the SPUD assay - no inhibitors were detected [36].
Tests for absence of brainstem reflexes and persistent apnoea had been carried out and patients were qualified for transplantation procedure.
All flake food is tested for absence of estrogenicity using the yeast estrogen screen assay (Routledge and Sumpter, 1996) prior to use.
In addition, calibration data were tested for absence of heteroscedasticity, since this would change the uncertainty of analytical results in a concentration dependent manner.
All cell lines have been tested for absence of mycoplasma contamination with MycoAlert (Lonza) -last control March 2013 -and authenticated by STR analysis using genRESVR MPX-2 and genRESVR MPX-3 kits (serac, Bad Homburg, Germany).
With intercept considered an artifact in this particular case, linear regression provided better fitting and is therefore considered the correct approach Additionally, calibration data were tested for absence of heteroscedasticity, since this would entail the uncertainty of analytical results to vary in a concentration-dependent manner.
We tried a series of algorithms, including our variants and those suggested by others, and found that none of the algorithms that used absolute length scales satisfied the test for the absence of correlations between a random independent set and a strongly inhomogeneous non-random set (Tests and comparison of different packages and Table 1).
Diagnostic PCR with gene-specific and hph primers (Supporting Information, Table S2) was used to test for the absence of the open-reading frame of the respective deleted gene (with wild-type as a positive control) and the simultaneous presence of the hph cassette in the purified strains (Colot et al. 2006).
As in note 3, the influence of market intermediaries on vertical price transmission could not be tested empirically for absence of adequate data.
