Free-flight systems for testing extinction learning are not equally established for honey bees, but relevant tools have been developed to test flight behavior towards the nest ("homing") [28] or artificial foraging sites [29].
We fear-conditioned 100 Long-Evans rats, attempted to extinguish fear the next day, and tested extinction recall on the third day.
Finally, to probe savings, an aspect of eyeblink conditioning that depends in part on the deep cerebellar nuclei (DCN), after the 12-day initial training period we tested extinction and reacquisition.
The possibility that the mode of sucrose delivery could have contextual properties in CTA through a "renewal test" after extinction and a latent inhibition experiment was further tested in Experiments 3 and 4. When active testing followed passive extinction, a CTA was afresh obtained in rats actively conditioned in active conditions.
Furthermore, when given a choice test after extinction training (Experiment 2), animals trained with 32% sucrose, preferred the flavored solution, but animals trained with 4% preferred the unflavored solution.
Instead the procedures must be carefully designed to conform to pure incentive rules and exclude alternative interpretations (e.g., test in extinction, preserve instrumental baseline unchanged, etc).
The main task design consisted of four stages: a brief training session (3 trials per CS), a devaluation test in extinction (4 trials per CS) (devaluation sensitivity test), an extended training session (30 trials per CS), and a final devaluation test in extinction (4 trials per CS) (habit test) (Table 2).
All rats received three trials per 30-min session with the same intertrial intervals as in Experiment 1. Testing continued for 6 days until performance in the three conditions merged (i.e., tested to extinction).
