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By performing test cultures of different duration, we found that BM-OCs cultured for 4 days and BC-OCs cultured for 3 days generated trenches of similar length.
The test cultures maintained in nutrient agar slant at 4°C were sub-cultured in nutrient broth to obtain the working cultures approximately containing 10 CFU mL-1.
Then the test cultures (Bacillus cereus and Escherichia coli) were inoculated properly onto the media.
In the test cultures, the cell proliferation, necrosis/apoptosis, and multicellular spheroids generation were evaluated.
The pre-culture was intended to give an amount of algae suitable for the inoculation of test cultures.
Test cultures of bacteria were grown in Nutrient broth at 37 °C in an incubator shaker at 160 rpm.
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Unlike teachers at low-performing schools, those at schools where most students pass the tests said their biggest concern was the psychological impact of the test culture.
Then the test culture (Bacillus subtilis and Escherichia coli) was inoculated properly onto the media.
Petri dishes were filled with the respective test culture agar for bacteria and Sabouraud for fungi.
The test culture was sprayed on the TLC plate and incubated at 37°C overnight.
Twenty-seven (40%) of the undiagnosed patients had at least one enterovirus test (culture or PCR) performed.
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