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In patients unable to produce a sputum sample without induction, the string test cultured by MODS yielded Mtb in of 9/11 (82%%) TB positive patients compared to 11/11 (100 %) with induced sputum.
In the sputum-productive group, time to positivity for sputum cultured by MODS was significantly faster than string test cultured by MODS (median 9 vs 15 days, p = 0.006).
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We tested cultured rat nucleus pulposus cells for proliferation and cell cycle distribution under exogenous TGFβ1 stimulation with and without putative pharmaceutical inhibitors.
We simply had not tested cultured neurospheres at intermediate time points because we focused our analyses on more definitive in vivo experiments at the intermediate time points.
All tested cultured pancreatic cancer cell lines constitutively expressed B7-H3 mRNA under normal conditions (Panc-1 541 transcripts ± 36.6, MiaPaCa-2 358 transcripts ± 17.5, SU86.86 743 transcripts ± 725).
Since the late 1960s, researchers have recognized the importance of testing cultured cells for possible mycoplasma infection, which can invalidate results; in the 21st century, STR profiling now deserves equal recognition and should become a routine procedure in research laboratories.
For cell viability tests, cultured SK-N-SH cells in 96-well plates were treated with TCDD for 48 hr, followed by the addition of MTT in PBS at final concentration of 0.5 mg/mL for 2 hr.
Considering previous reports (Acharya and Chaudhary 2012) to this test, we cultured and isolated across a range of pH value as described above.
Freeze-dried (0.4 mL) microorganism was aseptically transferred into the broth media in test tubes, cultured and propagated anaerobically in the broth media at 37 °C in an incubator, and then used in the syngas fermentation process.
Broiler rinses that tested positive on the screening test were cultured for Salmonella with standard methods (i.e., selective enrichment, plating, serologic and biochemical confirmation).
Serology was positive for Leishmania spp. by indirect immunofluorescence antibody test, with cultured promastigotes of L. donovani used as antigen (immunoglobulin G [IgG] antibody titer 1 1,024).
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