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Strains that had reduced metabolism (higher remaining levels) of the test compound of interest were analysed further to confirm the role of the target gene in the predicted metabolic pathway(s).
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Effects of test compounds on levels of gene expression, enzyme activity, and morphological endpoints were determined using one-way ANOVA followed by Fisher LSD multi comparison procedure, where appropriate.
To keep comparability to the previous setup, we used the same 25 test compounds of a target as in the previous experiments.
We assessed the properties of this HTS co-culture assay using a panel of test compounds of known activity.
Pharmacologists test compounds of interest using in vitro and in vivo models [ 11].
The EST was validated by using a set of 20 test compounds of known in vivo embryotoxicity.
Conversely, observing a neutral loss of 35.977 u was found at 92.2% specificity for the presence of a chlorine atom in the 2 036 test compounds of the tested NIST11 MS/MS spectra, indicating a neutral loss of HCl.
The eight concentrations ranged from 25 to 1000 µM, and the different conditions mapped to largely different positions on the PCA plot used above to show the 12 test compounds of the present study (Fig. 6a).
Moreover, the effect of the test compounds on the inhibition of tubulin polymerization in vitro was estimated.
Figure 11 Anti-proliferation effect of test compounds on HCT 116 was assessed by MTT-assay.
To examine the effects of test compounds on growth, triplicate epimastigote cultures were seeded with 1×10 cells·ml−1×10
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