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Each test compound was conducted in triplicate.
Toluene was used as the test compound.
The thrombolytic ability of the test compound is evaluated.
Tubes without any test compound were used as a control.
The test compound is injected prior to the thrombus formation.
Test compound was replaced by DMSO (7.5% final) as control.
This would indicate the anticoagulant nature of the test compound.
where Ablank is the absorbance of the control reaction (containing all reagents except the test compound) and Asample is the absorbance of the test compound.
After 12 h of incubation at 37 °C, the test compound (40 μM) was added.
As shown in Fig. 5, the absorbance decreased with increasing the concentration of the test compound.
Each test compound was evaluated at a 2 μg/mL level in distilled water.
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