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The involuntary culling protocol of this project facilitated relative expression of fitness among the test breeds under the prevailing production conditions.
The highest accuracy of imputation for all three test breeds was achieved when using BEAGLE with un-phased reference data (mean genotype correlations of 0.90, 0.89 and 0.87 for Holstein, Jersey and Nordic Red respectively) but IMPUTE2 with un-phased reference data gave similar accuracies for Holsteins and Nordic Red.
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A second objective was to test breed effects on estrus synchronization and superovulation responses.
Twenty-four sheep were used to test breed and age differences in voluntary intake (VI).
Twenty-four non-pregnant sheep, divided into four groups of six animals each, were used to test breed (Suffolk and Rambouillet) and age (mature (six years old) and yearling (16 months old) ewes) differences in micromineral status.
To validate the C57BL/6N- Tyr cWTSI line, Tyr cWTSI animals were test bred to chimeras from a new EUCOMM strain yielding black mice carrying the targeted ES derived allele while all albino offspring were wildtype (data not shown).
Because of favorable characteristics, such as abundant number, high polymorphism and co-dominant inheritance, microsatellite DNA markers have been extensively used for a number of applications in livestock genetics, including parentage testing, breed classification, conservation genetics and also to assess genetic variation and structure within and among populations [ 3].
Future work will test additional breeds, management conditions and viral subtypes, and identify functional properties of the haplotype this deletion variant tracks.
All comparison tests among breeds were two-tailed t-tests performed with the t.test function in R (v.3.1.0).
Statistical models included breed of dam as a fixed effect with year of entry and doe nested within doe breed as the error term to test doe breed.
He added that other aspiring operators had tested different breeds of amphibious vehicles, but that none had proved stable and powerful enough.
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