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Upon completion of the social preference test, brains from experimental animals were fixed using immersion fixation, sectioned at 30 µm on a freezing-sliding microtome, and then the free-floating sections were stained for ERα using standard AB immunocytochemistry (for complete details see [8]).
After Morris water maze test, brains were removed (n = 4).
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But we don't exactly have all the medical clinics necessary to test brain insertion".
TCD also can be used to test brain autoregulation and vasoreactivity to CO2[36].
It has been used for decades to test brain function, and has been approved for treatment of depression.
Three probabilistic brain atlases were also used to parcellate the test brain.
To compute such chance concordance distributions, we compared random parcellations of the test brain.
Several different procedures were used to create a set of distinct anatomical parcellations of the test brain.
An additional macro-anatomical parcellation specific to the single-subject test brain is available from the International Consortium for Brain Mapping (http://www.loni.ucla.edu/ICBM/Downloads/Downloads_ICBMtemplate.shtml).shtml
We analyzed the pair-wise spatial correspondences between anatomical regions defined in eight distinct parcellations of a single test brain, the ICBM single-subject template.
The test brain was then parcellated using FreeSurfer, and manually edited by an expert anatomist to correct any apparent gross mislabelings.
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