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The effect of AgNPs on anaerobic digestion was assessed using a biomethane potential (BMP) test as previously described [45].
Continuous secondary efficacy variables were analyzed using analysis of variance and categorical secondary variables were analyzed by Cochran-Mantel-Haenszel test, as previously described (Calabrese et al., 2014).
Shock sensitivity was evaluated following completion of the conditioned fear test as previously described [17].
The cells were then fixed with 2% PFA PBS for FSCAN test as previously described [36].
Exercise capacity was measured at peak exertion during a symptom-limited exercise-treadmill stress test as previously described[16].
Cell death of the target cells was studied by trypan blue exclusion test as previously described [10].
A PMLV-infected HeLa cell line was used as antigen in the WB test as previously described in detail [21].
DTH responses against P. brasiliensis antigens were evaluated 90 days after infection by the footpad test, as previously described [21].
All subjects had DEXA assessment of body composition and assessment of aerobic fitness using a submaximal VO2max test, as previously described [15].
Intestinal permeability is measured by the sugar absorption test, as previously described [ 18].
Maximum heart rate (HRmax) was determined during the test as previously described [ 25].
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