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Additional large studies aiming to validate this questionnaire as a screening or diagnostic test are needed.
Further studies using other design test, such as microshear test, are needed to confirm this hypothesis.
Therefore, further studies of LGMD cases confirmed by genetic test are needed to determine the correlations between genotype and serum enzyme levels.
However, more studies which include measurements as TDM or resistance test are needed to evaluate the use of this drug in paediatric population.
However, more studies with higher sample sizes which evaluate the usefulness of new tools like virtual phenotypic resistance test or the combination of pharmacokinetics parameters and resistance test are needed to improve the management of HIV-infected children.
Therefore, specific cost-effectiveness studies, including all management costs of the disease, survival and quality of life and presenting different applicative scenario of imaging techniques in the case of a positive AFP test, are needed to refine the comparison of CAI→US vs standard US surveillance.
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However, the allelism test is needed to distinguish the Bph21 gene from Bph18 or Bph10.
However, allelism test is needed to confirm the independence of Bph 20 and Bph21 genes.
A formal statistical test is needed to test the significance.
Further optimization and validation of the ELISA test is needed.
Otherwise, a permutation test is needed.
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