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Cytocompatability of the scaffolds was assessed using MTT assay, direct contact test and cell attachment studies.
Cytocompatability of the composite scaffolds were assessed by MTT assay, direct contact test and cell attachment studies.
Furthermore, the stability and biocompatibility are confirmed by numerously repeated usage test and cell proliferation and attachment examination, respectively.
Cell adhesion was determined quantitatively by LDH total test and cell proliferation was assessed by confocal microscope observation.
Cytotoxicity test and cell morphology analysis confirmed that adhesion and proliferation of osteoblasts on CMCS-modified Mg alloy surface were improved remarkably.
Characterisation of the prepared nanopowder was carried out by XRD, FTIR, TEM, SEM, EDX, ICP, zeta potential, acid dissolution test, and cell culture test.
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Cell cultured medium was collected for MTS cytotoxicity test and cells were harvested for proteasome activity determination.
To determine cell proliferation, the growth rate was evaluated by both MTT test and cells counted.
Batteries of molecular tests and cell cultures allow detection of a widening range of toxins, for a fraction of the time and cost of traditional animal tests.
Scaffolds loaded with PLGA nanoparticles were subjected to physico-mechanical and biological characterizations including morphological analysis, swelling and dissolution tests, mechanical compression tests and cell viability tests.
PCTBV-25 was also shown as a safe material with good biocompatibility by cytotoxicity tests and cell growth experiments.
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