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Below a depth of about 4,500 metres, which is the carbonate compensation depth (CCD), the pressure and temperature of seawater produces a rate of dissolution in excess of the rate of pelagic test accumulation.
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There was a lack of studies testing accumulation and pathway effects.
Guidelines for achieving this cover three aspects of surrogates testing: experimental design of tests, ongoing corroboration assessment of evidence produced by tests, and accumulation of lessons learned from multiple test studies over time.
The intestinal transit test showed accumulation of the pellets at the anorectal junction.
Given the analytical precision of our test, relevant accumulation in plasma if present would have been detected.
However, a limitation of our study is, we did not test for accumulation of other organelles and protein aggregates.
To test this, accumulation of ciprofloxacin in strain BB255 grown with and without diclofenac was measured fluorometrically.
To test whether accumulation of DRIPs occurs and correlates with decreased SG formation, we labeled nascent chains using O-propargyl-puromycin (OP-puro).
The genes were cloned into a panel of Agrobacterium tumefaciens binary plant expression vectors in order to test HA accumulation in different cell compartments.
To test whether accumulation of MIZ1 is required for repression of MYC target genes, we inhibited HUWE1 in control cells and in cells that express a doxycycline-inducible shRNA targeting MIZ1 (Fig 7A).
To test for accumulation of AMPARs in REs, we assayed whether TC10 mutants increased endogenous AMPAR co-localization with TfRs, which largely exist in REs in dendritic shafts where they co-localize with GluA1-containing AMPARs (Carroll et al., 1999; Ehlers, 2000).
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