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When treatment standards require the removal of plant nutrients from the sewage, it is often done as a tertiary step.
The children referred to our pediatric neurology clinic, which is a tertiary step medical center in our country, with chronic or recurrent headaches (n = 310) were prospectively evaluated between February 2001 and February 2004.
When a tertiary step was necessary, the plates were washed and incubated for 1 h at 37°C with HRP-conjugated component (antibody or streptavidin).
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These secondary and tertiary steps were performed at room temperature for 30 minutes.
To determine the irritation threshold, n-butanol (Sigma-Aldrich) was diluted into PEG 200 and placed into glass bottles in an 18 tertiary-step dilution.
Further research is needed to investigate the feasibility of adding a tertiary treatment step to increase the nutrients and microorganisms removal from grey water.
Whereas, for example, the Swiss Federal Government started projects introducing a tertiary treatment step at many of its WWTPs, the discussion whether additional wastewater treatment technologies are ecologically worthwhile is still ongoing [9].
Wetlands can be used as a secondary or tertiary treatment step, following chemical and/or biological treatments, and rely upon natural processes in shallow water or temporarily flooded land that is able to support aquatic life [18].
In Sweden, hospitalization for type 2 diabetes may be a secondary or tertiary referral step; type 2 diabetes is diagnosed in primary care centers, which refer the patients to hospital outpatient clinics or directly to inpatient clinics.
Finally, the environmental risk posed by pharmaceutical residues in effluents from constructed wetlands acting as secondary and tertiary treatment steps is calculated in the form of the risk quotient ratio.
(3) In the secondary and tertiary PCR steps, the target DNA should be exponentially amplified via high stringency cycles, while non-target products are not amplified by the gene-specific and FP-specific primer pairs.
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