Sentence examples for tertiary constraints from inspiring English sources
Second, we performed low-resolution modeling using the pre-assembled helices and tertiary constraints derived from maxima in the 2D MOHCA-seq proximity map.
Tertiary constraints were determined by identifying peaks from analyzed data that were (1) distinguishable from the local background signal by unbiased inspection and (2) not attributable to secondary structure, based on secondary structures inferred by M (Kladwang et al., 2011a).
This retention of local tertiary constraints is supported by HDXMS data for the peptide containing residues 241 251 that is not fully exchanged even at 4 M GdmCl, indicating the presence of some hydrogen bonding in β sheet B and the lack of complete PEGylation at 3 M GdmCl for the Cys residues introduced at positions 55 and 65, near Ser47, and at position 237, near Cys232.
The results also suggest that in the absence of significant tertiary structure constraints double variants that preserve pairing in stem regions will typically be accepted.
It thus provides more realistic structure predictions for peptide segments submitted to tertiary and quaternary structures constraints i.e., within the protein environment.
The residues contact map provides spatial constraints for constructing tertiary structure models of TMH proteins, which has recently been a hot topic in protein structure prediction [12, 13, 14, 15].
LOMETS is a local threading metaserver, for protein tertiary structures predictions and spatial constraints [ 53].
In general, β-sheets and loops are more ambiguous than α-helices since their tertiary structures generate fewer edge constraints.
Other models explore the interdependence of sites due to constraints introduced by tertiary structure in protein sequence evolution [ 19- 21].
Because the lack of tertiary structure removes purely architectural constraints on conservation, alignment of intrinsically disordered regions tends to highlight functionally important residues.
The results show that the SAXS constraints complemented the tertiary-structural information for all the proteins, and that accuracy of the structures thus obtained with SAXS constraints and local geometrical restraints ranged from 1.85 to 4.33 Å.
