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The tertiary amplification fragments were visualized through electrophoresis in 1% agarose gels, where the average fragment size was usually between 300 and 500 bp.
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Due to low titre, fluorescent FREM1 detection in paraffin sections using rat monoclonal antibody required tertiary signal amplification first using rabbit anti-rat-Alexa-Fluor-488 followed by donkey anti-rabbit Alexa-Fluor-488 and donkey anti-rat-Alexa-Fluor-488.
TSPs 1, 2 and 3 (Supplementary Material, Table S2) were designed to be used with DNA-walking ACP primer and Universal primer for primary, secondary and tertiary genome-walking amplification.
The Aub protein is posited to work within the ping-pong cycle by binding tertiary piRNAs that are generated through the amplification loop.
For signal amplification, slides were washed and incubated with appropriate tertiary antibodies for 2 hours.
(methyl tertiary butyl ether).
(Answer: amplification).
OF AMPLIFICATION about asbestos.
(No amplification is used).
TWO: No amplification needed.
OF AMPLIFICATION about Saint Brendan.
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