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It is also a terrific medium for sautéing fruits.
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Terrific broth medium was chosen for optimal cell growth based on a significantly increased cell growth compared to Luria Bertani medium.
The prime Angus burger ($9) was terrific, cooked medium rare as ordered and running with juices.
Terrific Broth medium (TB), with 0.1% carbenicillin and 0.1% glucose, was used for the gene expression under controlled temperature (37 °C).
The optimization of induction process conditions led us to perform the recombinant protein induction at 25 °C for 16 h, with 0.1 mM IPTG in Terrific Broth medium.
For expression of GST-tagged MtbHadAB complex, cells were grown in Terrific Broth medium supplemented with 100 μg/mL ampicilin and 50 μg/mL kanamycin at 37°C.
Each culture was then diluted 1 100 with Terrific Broth medium with 50 μg/ml kanamycin (pH 6.4) and cultured at 37 °C, 200 rpm until the OD600 of bacteria culture was 0.8.
The resulting culture was diluted (1 25) into 100 mL of terrific broth medium in a 500-mL flask, and IPTG was added to a final concentration of 0.01 mM to induce protein expression.
E. coli were transformed with the plasmids and grown in 3 l Terrific Broth medium.
TB (Terrific Broth) medium plates (III) showed better growth conditions than other two types of media.
Protein was overexpressed in E. coli BL21 DE3) CodonPlus-RIL cells (Stratagene) grown in terrific broth medium.
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