Sentence examples for terrific broth from inspiring English sources
Cultures were diluted to OD600 of 0.1 using either Terrific Broth or Terrific Broth supplemented with 1 mM IPTG.
The optimization of induction process conditions led us to perform the recombinant protein induction at 25 °C for 16 h, with 0.1 mM IPTG in Terrific Broth medium.
New liquid cultures (Terrific Broth) were inoculated and allowed to grow at 37 °C to OD600 of 1.0 1.2.
Protein expression was conducted in E-coli strain BL21 (New England BioLabs, Ipswich, MA) in terrific broth medium.
Cultures of BL21 DE3)/pDEST17-HiDXS were grown at 37 °C in Terrific broth containing 50 μg ml−1 ampicillin until OD600 nm=1.5.
This revealed that RN1008 was unable to form colonies on LB, 2xYT, and Terrific Broth plates for at least seven days (Fig. 4).
Recombinant constructs were transformed into E. coli BL21 cells (Novagen) and selected clones cultured in the Terrific Broth medium with ampicillin (100 µg/ml).
Terrific Broth medium (TB), with 0.1% carbenicillin and 0.1% glucose, was used for the gene expression under controlled temperature (37 °C).
A freshly transformed single colony of each clone was inoculated in 10 ml Terrific Broth (TB) containing 100 μg/ml (1×) ampicillin and grown over night.
For expression of GST-tagged MtbHadAB complex, cells were grown in Terrific Broth medium supplemented with 100 μg/mL ampicilin and 50 μg/mL kanamycin at 37°C.
