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In adipose tissue we identified more upregulated GO terms compared to liver tissue (106 vs. 36) and for the down-regulated GO terms we detected 2 and 19 in adipose tissue and liver tissue respectively.
For both down- and up-regulated GO terms, we detected an intersection that included between 25% and 80% of the N. furzeri terms and that was particularly extensive for up-regulated DEGs (Table 1).
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In HSPB1∆C-term fibroblasts we detected bands corresponding to all possible combinations of HSPB1 dimers.
In both the constant condition experiment and the short-term stress experiment, we detected no significant effect of Df 3R ED5579/DSK001 or Hsp70Ba304/Hsp70Ba304 on FA of any trait after Bonferroni correction of probabilities (Table 6 and Table S3).
Accordingly, while we did not find additive sire effects at 27°C for any of the male traits measured (as indicated by the Sire × Treatment terms), we did detect additive genetic variance for all male traits at 27°C using the Biomodels.
By long term follow up we detected a 5% difference which whilst a statistically significant improvement did not reach our threshold for clinical significance.
In terms of cellular compartment we detected changes mainly in the cytosolic or nuclear compartment (Supplementary Tables S4 and S5).
In terms of genomic features, we detected T2D-associated differential DNA methylation mainly in LCP and ICP, while HCPs are underrepresented.
In terms of taxonomic composition, we detected Escherichia and Bacteroides as the most abundant organisms in the 1-month-old infant, whereas Bifidobacterium only appeared and grew to dominate the infant GIT microbiota at some later stage between 1 and 11 months.
For long-term potentiation (LTP) measurements, we detected no differences in fEPSP slope among WT, APP/PS1, RanBP9+/−, and APP/PS1; RanBP9+/− slices at baseline.
However, in a sufficiently powered mixed model analysis of IL-6 and HA RNA data including study year as a term in the model, we detected no significant year effects (p = 0.61 and 0.47, respectively).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com