Local soil conditions are expressed in terms of amplification factors.
The performance of the real-time PCR assay was evaluated in terms of amplification efficiency (92%).
This paper reviews wind-tunnel and CFD techniques to determine PLW speeds expressed generally in terms of amplification factors defined as the ratio of local mean wind speed to mean wind speed at the same position without buildings present.
The regions differed in their ability to discriminate species, and in ease of retrieval, in terms of amplification and sequencing success.
The JB3-JB5 primer set clearly outperformed the degenerated JB2-JB5GED primer set in terms of amplification success and lack of aspecific products (Fig 1).
Herein, we seize the opportunity to compare the I3M11 partition to the M1M6 partition in terms of amplification success and substitution pattern, as well as to assess the potential of their combination for species level delineation.
The taxon specific primers for the I3-M11 partitioutperformedmed the universal M1-M6 priners in terms of amplification success (87.8% vs. 65.8%, respectively) and produced a higher number of bidirectional COI sequences (65.8% vs 39.0%, respectively).
First, in terms of amplification efficiency, all primer sets seem acceptable because they have similar and close to perfect amplification efficiency of 2.0 (Table 2).
From these five candidates, the two best-performing gene models (in terms of amplification efficiency via RT-qPCR and closest-to-equivalent expression) were chosen as control genes for this study.
In terms of amplification, there was indeed an agreement between CISH and FISH in 96% of the tumours, which is almost the same as the agreement previously published (93.6%) (Tanner et al, 2000).
There are many examples from the current high throughput sequencing experiment of sequences that have the same nucleotide content (and therefore, the same GC content), but vastly different outcomes in terms of amplification success due to the actual arrangement of the nucleotides in the sequence.
