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Acetylation of the N terminus was achieved with excess acetic anhydride and DIPEA in DMF.
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As shown in the present paper, immunoprecipitation efficiency of about 40 50% of available DNA termini was achieved with the dDIP technique, which suggests a better efficiency most suitable for the capture of rare damage events.
Accurately tailored 5' and 3' termini are achieved by positioning RNA Polymerase I (Pol I) transcription initiation and termination sites respectively (or by using the Hepatitis Delta virus ribozyme to cleave the correct 3' end).
Mutation of the 2 potential nuclear localization sequences at the C-terminus of Rac1 (rac1-5Q) was achieved by site-directed mutagenesis on pExp-PADH1GFPRAC1, resulting in the mutation of K223KRKIKR229 to Q223QQKIQQ229.
Deletion of the N-terminus of RFA2 was achieved by PCR amplification of RFA2 cDNA from pJM218 with a primer in which the codon for amino acid 39 was replaced with a start codon contained within an NcoI site.
Deprotection of the Fmoc group from the N-terminus of the sequence was achieved with 2 cycles (7 min each) of a piperidine solution in DMF (70/30 v/v).
Trimerization of scFv l αhCD70 was achieved by fusing the C terminus of the scFv to the trimerization domain of tenascin-C (TNC).
Display of the targeting moieties on the viral surface was achieved through fusion to the N-terminus of gp64, the major envelope protein of the Autographa californica nuclear polyhedrosis virus (AcNPV).
The fusion of Atg4 to the C terminus of Cot1 (Vac), Snq2 (PM), Msp1 (Mito) and Ant1 (Pex) was achieved through homologous recombination.
This was achieved by adding a homopyrimidine sequence to the 5′-terminus of a PCR primer.
Targeted degradation of Yae1 and Lto1 was achieved by fusing a photosensitive degron (psd) to their C-termini.
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