High solid and thermally stable waterborne polyurethanes (WPUs) were designed and synthesized by introducing imide groups into the hard segment and locating the anionic groups at the chain termini (terminal ions).
We previously isolated the gene encoding HRP C1a and showed that HRP C1a is at first translated as a preproprotein containing propeptides at its N- and C-termini (N-terminal secretion signal peptide and C-terminal propeptide; CTPP).
ProRS (orf19.6701), in addition to catalytic and anti-codon binding domains, had two additional domains one at its termini: N-terminal (editing domain) and a C-terminal domain.
mCD36ED DNA, amplified by PCR with a hexa-histidine tag at its carboxyl-terminus (C-terminal His-tag), was cloned in a baculovirus transfer vector and mCD36ED protein was expressed using a baculovirus/insect cell expression system.
Its amino acid sequence is well conserved at both terminuses (N-terminal, 65 100 % similarity (~220 amino acids); C terminal, 75 100 % similarity (~900 amino acids)).
After alignment of these fragments with the CLV3 proprotein, two most frequently occurring termini, N-terminal Met39 and Arg70, were detected, suggesting internal cleavage sites located before Met39 and before Arg70.
Other than its transactivation domain, which resides at its N-terminus, the C-terminal aminoacids also play a major role in its activity.
When recruited to 40S pre-ribosomal particles via the C-terminus, the N-terminal part seems then to facilitate the U3-dependent processing of the 18S rRNA precursor.
The confocal microscopy results of DND1 and APOBEC3 localization were identical when GFP or mCherry were cloned either as N-terminus or C-terminal fusion proteins to DND1 or APOBEC3, respectively (data not shown).
This was not surprising since caspase-3-like enzymes cleave APP at its C-terminus, and C-terminal APP-Gal4 fragments are the ones detected by our luciferase assay.
