These findings indicate that the terminus site of DNA replication is a special region, extending for several hundred kb and including loci such as T1, T2, tus, etc.
The Ori MD contains the origin of replication and is located opposite the Ter MD, which contains the replication terminus site.
The C-terminus sites s7 and s6 are critical for the endoplasmic reticulum retention and intracellular disposal.
However, the C-terminus glycosylation sites were more exposed to the mannosidase cleavage in the ER than the N-terminus sites.
This model predicts few short-lived rhodopsin activations, since arrestin-1 would rarely bind until most of the C-terminus sites had been phosphorylated.
Aβ peptide has a β-sheet forming core starting from residue number 16 and extending to the C-terminus; thus, inserting a dye of a size comparable to five amino acids at the center or C-terminus sites may affect fibril formation.
In vitro studies and in vivo studies in small animals, baboons and macaques show that anti-Aβ antibodies are generated with the expected N-terminus site-specificity, and that these antibodies have functional immunogenicities to neutralize the toxic activity of Aβ and promote clearance of plaque deposition.
Subsequently, the membranes were incubated for 3 h with a 1∶200 dilution of rabbit polyclonal antibody against amino acids 848 865, which corresponds to the C-terminus site of CaL channel α1C-subunit (Alomone Labs, Jerusalem, Israel).
Yang et al. [ 14] reported that helitrons could recognize a new 3'- or 5'-terminus site to form a new element in A. thaliana.
Upon ubiquitylation, photolysis cleaves the ligation handle, thereby restoring a native ubiquitin C-terminus, site-specifically attached to a single lysine residue.
