Due to the fact that attachment of the tag to the actin C-terminus may cause problems in its folding (Brault et al. 1999; Rommeleare et al. 2004), we chose to attach AcGFP to the N-terminus of actin preceded by linker (16-amino acid rests).
The railway terminus was not built, and problems with drainage on the approach road made access to the bridge difficult for vehicles.
A possible solution to the problem of aligning termini to be included in this analysis would be to assume that the respective motifs for posttranslational modifications are recognized as extended peptides followed by some linkers of varying length.
But the second episode of Season 5 proved that the problems that started at Terminus are far from over.
This road, which runs between the famous Victoria Terminus and Crawford Market, encapsulated all the problems of conservation in Mumbai.
First, let me say that we are fully aware of the congestion problem that exists at the northern terminus of the 710 Freeway in the Alhambra area.
The use of primers situated near the genomic termini during the cDNA synthesis stages have been shown to improve the number of termini sequences, but have not completely resolved the problem.
Indeed the introduction of non-native amino acids at the N- terminus is not ideal and it is a common problem that generally exists both for the LIC and Gateway technologies.
A β-capping motif was previously designed to help solve this problem, but not without limitations, as the termini of this β-cap were not fully available for chain extension.
The problems are most severe at the southern terminus, but it is not the only section of the road with issues.
