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This light chain can interact through the N and C termini with actin and myosin heavy chain.
pIXs were modified at their carboxy termini with a Flag sequence, a hexahistidine sequence (His6) or a monomeric red fluorescent protein (mRFP1), respectively.
Active SERCA1a and WT-PLB, tagged at their N termini with fluorescent proteins (CFP and YFP), were coexpressed in stable HEK cell lines, and fluorescence resonance energy transfer (FRET) was used to detect their interaction directly.
Furthermore, a perturbation influences the geometry of the TM helix, mediates inter-helical interactions within and across protein chains and avoids hydrophobic mismatch of the helix termini with the bilayer.
Here we report two bulky G2 dendronized norbornene macromonomers 8 and 13 containing nine hydrophilic triethylene glycol (TEG) termini with three and nine, respectively, 1,2,3-triazole (trz) rings, and their controlled polymerizations via the ring-opening metathesis polymerization (ROMP) technique using Grubbs' third-generation olefin metathesis catalyst.
However, the change of route actually increased the length between the two termini with 23 km.
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We capped all such protein chain ends, including the N- and C-termini, with hydrogens to form neutral NH2 and COOH groups.
Upon higher-energy collisional induced dissociation (HCD) SPITC peptides in ESI generated predominately y-type ion series; such simplification of spectra enables the identification of N-termini with high confidence.
Green fluorescence protein (GFP) was genetically fused at its N- and C-termini with two sortase recognition sequences pentaglycine and LPETG, respectively to yield a linear GFP (l-GFP).
Proteins are named according to their N- and C-termini with the nomenclature of Campbell et. al. [30].
The constructs fell into two broad classes: i) large insertions into the split site (constructs 1 and 3) or, ii) circularly permuted (swapping of the N- and C-termini) with or without insertions (constructs 2 and 4).
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