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The same approaches allow crystallographers to rapidly determine the quality of their protein and rationally engineer the deoxyribonucleic acid (DNA) construct to remove microheterogeneities and flexible termini that are not visible by classical staining of sodium dodecyl sulfate (SDS -gels.
Accordingly, the design of dimerization inhibitors should not focus only on the flexible N and C termini that constitute most of the dimer interface, but also on other structured regions of the monomer.
These RNAs are distinguishable from siRNAs by their longer length (24 29 nucleotides, nt) and by the lack of the 2',3' hydroxyl termini that are characteristic of miRNAs and siRNAs.
Of 74,135 available ESTs, 3,129 had 5′ termini that mapped upstream of the predicted start codon.
We then reversely analyzed the proportion of rice snoRNA 5′ termini that could be precisely captured in the degradome.
The 98 substrates were subjected to visual inspection to identify the region of the carboxyl termini that had the E Block (Table S3).
Similar(27)
Cumulatively, these changes may explain the different specificities/affinities of FGF18 and FGF8b, however, it is likely that differences in the N- and C-termini that are not resolved in the FGF18 crystal structure, contribute substantially.
Mammalian IRP2 molecules contain a unique conserved insert of 73 amino acids close to their N-termini, that is absent in IRP1.
Since both subunits have high arginine content and multiple proline-rich repeats at their C-termini, that are often intrinsically unstructured [49], this propensity to dissociate likely arises from their C-terminal tails.
Members of the PAPS3 family of plant poly(A) polymerases are smaller than the other poly(A) polymerases, lacking the extended C-termini that include putative nuclear localization sequences (Figure S1).
The Asp173-Gly174 was chosen as an insertion site, because it is located at the opposite side of N- and C-termini that would allow tags to work effectively by minimizing steric hindrance provided by the fusion partner (Fig. 1A).
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