In addition, proper methylation of a specific lysine residue in a particular histone often depends on histone termini of the same or another histone (Fingerman et al. 2008; Lee et al. 2010).
Here, dots and rods of the same colour represent intact ethyl moieties and ERC-reacted ethyl termini of the same molecule, respectively.
The crystal structure of LcrV, which shows that the molecule is shaped like a dumbbell with both N- and C-termini on the same side (Figure 3D) [42], indicates that both motifs are exposed on the protein surface, thus being potentially available for interactions with other protein partners (Figure 3D).
To investigate the effect of rotation of the dipole along two axes, we tested cp195, which has its N-terminus on the same side of the barrel as wtVenus, and cp173, the N-terminus of which is on the opposing side of the barrel.
SpREJ2 and 7 are the only SpREJ proteins predicted to have both N- and C-termini on the same side of a membrane.
The C-terminus of rifin, but not that of PfEMP1, prevented GFP export by Type 1 N-termini, including the N-terminus of the same rifin protein.
This treatment labels only the lower band containing the polypeptides with externally exposed C-termini, thereby demonstrating that the N- and C-termini are exposed on the same side of the membrane, as anticipated by the Fluc hydropathy profile.
Since mTq is inserted and the N and C termini of mTq are on the same side of the β-barrel, we reasoned that a C-terminal deletion of six amino acids (mTqΔ6, yielding a fluorescent mTq as in yellow cameleon [ 31]) would effectively bend the mTq moiety in Gαq, thereby forcing the mTq fluorophore (and its dipole moment) in another orientation.
FRET in mobile oligomers and large aggregates were only observed when the FPs were on the same termini of the polyQ construct (polyQ-CFP plus polyQ-YFP, or CFP-polyQ plus YFP-polyQ) and not on different termini (i.e., polyQ-CFP plus YFP-poly Q. YFP-poly Q
