Sentence examples for termini coding from inspiring English sources

In contrast to the PDE4 splice variant 5' exons (amino termini), coding regions found in the LR1, LR2, and terminal catalytic domain exons are highly conserved across species (Additional File 2).

As described above, addition of an N-terminus coded by the attB sites to the ceQORH protein has no impact on the production of this recombinant protein when produced in L. lactis (figure 3A).

However, only in the case of the L. hesperus MaSp1 and MaSp2 that we report here, and the full-length CySp1 and CySp2 cDNAs from Argiope bruennichi, is it certain that the N- and C-termini coding regions belong to the same gene.

It has been proposed that the C-terminus coding extension of the COX2 protein (Mcox2e), which is unique to freshwater mussel M genomes [32] [36], could represent an "M-specific label" for sperm mitochondria that determines their fate in the fertilized eggs (as observed in Mytilus [37], [38]).

Therefore, the first nucleotide at the 5' terminus of coding transcripts or of RNA genes is biased to undergo sequencing, since no hydrolysis is required to obtain a read beginning at this position.

The normal function of the N-terminus code may be to help "hide" the embryo from the mother's immune system, the team suggests in the 12 July issue of Cell.

Sequence drift appears to be responsible for the development of PDE4 amino termini-coding exons, as well as promoter development.

The Aqp1b-Ct1a chinera, in which the C-terminus of Aqp1b was replaced by that of Aqp1a, was obtained by amplifying the C-terminus-coding nucleotide sequence of Aqp1a with the primers 5'-CACGAGCGGACGACTTCCCCGAGCGC-3' and 5'-ACTAGTCGTCTGTGTGGGACTATTTTGACG-3'.

To obtain the Aqp1a-Ct1b, in which the C-terminus of Aqp1a was replaced by that of Aqp1b, the C-terminus-coding nucleotide sequence of Aqp1b was PCR amplified using a forward primer partially complementary to Aqp1a, 5'-CCCCCAAATTCCAAAACTTCAGGACGCGCAG-3', and a reverse primer bearing a SpeI restriction site, 5'-ACTAGTGCTTGTTTTTTCAGTGCTTTGG-3'.

Sequences identified with a predicted methionine at the N-terminus were coded with '(M)'.

Therefore, we used the BacMagic baculovirus expression system by placing a His tag at the N- terminus of the coding region of the genes to allow for rapid purification of over-expressed proteins by a Probond purification kit (Invitrogen) under denaturing conditions according to the manufacturer's instructions.