21 The cluster of 4-helix bundles, R2 R4, has a markedly different topology, with the N- and C-termini at the same end of the bundle.
The Cd statistic can range from 0, in cases where there is equitable division of the genome, to 1, in cases where the origin and terminus are at the same chromosome location yielding essentially a single replication arm.
Supporting our conclusions, in a crystal structure of ROCK-I with RhoE lacking its N terminus, which we determined at the same resolution in the same space group, no electron density is observed at the phosphoacceptor site (data not shown).
Tagging dmNnf1R-1 athehe amino-terminus and dmNnf1R-2 athehe carboxy-terminus produced the same localization pattern (data not shown).
(A ) Tracking of the scattering signal from the gold nanoparticle attached to the N-terminus (black) and fluorescence signal from the GFP moiety located at the C-terminus of the same myosin 5a molecule (green).
Ion channel proteins are enriched for disordered residues at the N-terminus, and the same is true to a lesser extent for C-terminal disorder.
Interestingly, A-13 and C-16 are in homologous locations on their respective chains at the amino termini, binding to the same receptors and have similar activity (Ponce et al, 2001; and unpublished data).
A comment containing the word TAG; e.g., "HIS TAG" The N-terminal or C-terminal sequence of a chain was identical in sequence to that of an annotated tag (at the same terminus) that was at least 5 residues long, OR the entire chain sequence was identical to another PDB chain annotated with tags.
The 4-helix up-down-up-down fold results in the N- and C-termini of the domain being at the same end of the bundle (Fig. 3A), whereas with a fifth helix the N- and C-termini are at opposite ends of the bundle.
In a similar way to the aspartates that interact with N-terminal regions of α-helices, the charged residue, arginine, has the highest propensity to form capping interactions that are both conserved and buried at the C-termini of α-helices, while at the same time compensating for the helix dipole (Table 2 and see Additional file 3, Figure S1B - grey bars).
