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Specifically, four different termini (i.e., 5TSS, 3TSS, 5TNS and 3TNS) coexist in this sequence read where they form a "double-termini assembly".
The long form transcripts consist of exons encoding the amino termini (i.e., 5' exon), upstream conserved region-1 (UCR-1), linker region-1 (LR-1), UCR-2, LR2, catalytic, and carboxy-terminal domains.
FRET in mobile oligomers and large aggregates were only observed when the FPs were on the same termini of the polyQ construct (polyQ-CFP plus polyQ-YFP, or CFP-polyQ plus YFP-polyQ) and not on different termini (i.e., polyQ-CFP plus YFP-poly Q. YFP-poly Q
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Then, two site-directed techniques were explored to increase column efficiency by immobilizing the antibody via its C-terminus, i.e., away from the antigen-binding site.
Moreover, the addition of two separate hexameric histidine tags at both the amino and the carboxyl-termini (i.e. NC-6xHis-tagged Del-38) increased the binding affinity of the recombinant enzyme to the nickel-agarose.
The recombinant aromatase without the first 38 amino acids from the amino-terminus (i.e. Del-38) was found to have a higher activity than the full-length enzyme.
The natural N- and C-termini, i.e., the given order of secondary structure segments, are critical for protein folding and stability, as shown by several studies using circularly permuted proteins, mutants that have their N- and C-termini linked and are then digested at another site to create new termini.
To deconstruct the role of the different regions of aS sequence in this process, we measured the high-pressure dissociation of amyloids formed by covalent chimeric dimers of aS (syn-syn) and by the aS deletion mutant that lacks the C-terminus, i.e., aS (1 99).
It contains two BRCT domains at the C-terminus, i.e., phospho-peptide binding modules also carried by several proteins involved in the DNA damage response.
In vitro, APT-1 can depalmitoylate structurally different proteins (soluble or integral membrane proteins), having different subcellular distributions and S-acylated either at the N- (i.e. endothelial nitric-oxide synthase and Giα1) or at the C-terminus (i.e. H-Ras) [18].
Bypass of an adducted template would position the adduct opposite the primer terminus (i.e., insertion/translocation prior to extension).
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