PCR products were sequenced at the Australian Genome Research Facility AGRFF, Brisbane, Australia) using dideoxy dye chain-termination technology (Applied Biosystems).
Reversible termination sequencing technology was first reported by Dr. Jingyue Ju from Colombia University [13].
Reversible termination sequencing technology is a sequencing-by-synthesis approach [3 5] that infers the sequence of a template by stepwise primer elongation.
Due to various advantages, reversible termination sequencing technology has been widely accepted and used in multiple NGS platforms, including the most successful NGS, Illumina/Seloxa sequencer and the first third-generation single molecule sequencer, Helicos sequencer [3,4,12,19].
The EST database generated by 454 pyrosequencing was compared with that established by random sequencing of clones from an elicitor-treated opium poppy cell culture cDNA library using dideoxy chain-termination (Sanger) technology [ 1].
The chapter introduces state-of-the-art electro-optical circuit board fabrication technologies, termination, and performance demonstration for optical waveguides made of polymer and glass.
The standard approach to establish genomics resources for non-model plant species involves the random generation of expressed sequence tags (ESTs) from a cDNA phagemid library using dideoxy chain-termination (Sanger) sequencing technology.
In pharmaceutical companies, CEAs are mostly conducted for marketing and reimbursement purposes versus research and development, despite the fact that economic factors are usually considered the second leading cause for research termination of an early technology [26, 27].
After-location code is only used if the technology demands certain terminations after the work is done.
In this study, we report the integration of data from Sanger-based chain termination and 454 FLX pyrosequencing technologies.
