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Secondary structure predictions revealed 32 csRNAs exhibiting two stem-loop structures similar to the csRNAs known from S. pneumoniae, 16 with only the termination stem-loop, and 13 with more complex structures.
Overexpression of garlic FT2 in Arabidopsis resulted in an early-flowering phenotype and early termination of stem development.
In Arabidopsis thaliana, the HUA ENHANCER3 (HEN3), which encodes CdkE, a homologue of Cdk8, is involved in the proper termination of stem cells in the floral meristem and in the specification of stamen and carpel identities [13].
While zll alleles isolated in the Ler background show premature termination of stem cells [ 8, 13], putative null zll alleles in the Col background, such as zll ago10-1 [ 11, 18], have no or minimal effects.
Also see Figure 4 figure supplement 1. HCP: high CpG density; ICP: intermediate CpG density; LCP: low CpG density; TSS: transcriptional start site; TTS: transcriptional termination site; EpiSCs: epiblast stem cells; H3K27me3: histone H3 lysine 27 trimethylation; H3K9me2: histone H3 lysine 9 dimethylation; FC: fold change; GO: gene ontology; WGBSeq: whole genome bisulfite sequencing.
Daylength also influenced stem termination after flowering in soybean.
Previous research showed that stem termination affected both stem elongation and maturity in soybean, and the gene determinate stem 1 (Dt1) plays a primary role in soybean determination [ 16].
In Arabidopsis, AGO1 and AGO10 regulate the termination of floral stem cells through two microRNAs, miR172 and miR165/166 [ 10].
Stem termination in soybean is known to be controlled by at least two genes, Dt1 and Dt2, of which the former has a much greater effect [ 32].
Therefore, post-flowering photoperiod growth in soybean, assessed as maturation and stem termination, varied among the accessions tested and with the maturity genotype.
Flowering in e3/ e4 plants is followed by normal seed maturation and stem termination, possibly due to downregulation of Y and Dt1, respectively.
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