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The mass loss of composting mixture at termination of experiment was calculated by gravimetric procedure on oven-dry weight basis (Frederick et al. 2004).
At the termination of experiment, animals were sacrificed and tumor tissues were harvested.
The treatment was initiated one day before infection and continued till termination of experiment.
The treatment was subsequently continued at 3-day intervals until the termination of experiment.
Mitochondrial activity measurement was initiated by the addition of 5 mM succinate and 1 mM ADP. Rate of oxygen consumption was monitored for 5-8 minutes before termination of experiment with 1mM KCN.
Rh-123 (2 μM) was added 30 min before the termination of experiment.
Similar(48)
Humane end points refer to 'criteria that allow early termination of experiments before animals experience significant harm while still meeting the experimental objectives' (NC3Rs, 2013).
At termination of experiments, animals were bled under terminal anaesthesia then killed by cervical dislocation.
Premature termination of experiments affected the outcome.
Exchangeable Ca, Na, K and pH were determined after the termination of experiments (Table 3).
Due to mortality and premature termination of experiments, cumulative scores were not compared at 48 h in septic animals.
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CEO of Professional Science Editing for Scientists @ prosciediting.com