However, WRKY3 transcripts in the transgenic plants exhibited smear patterns on the RNA blot, which could result from premature termination of transcription, alternative splicing and/or degradation of WRKY3 transcripts.
Recently, the hMPV G gene was shown to be highly variable, particularly in the extracellular domain, as a result of nucleotide substitutions, insertions, and the use of alternative termination transcription codons (17– 17).
The results provide indication of alternative termination of the transcription in the C57BL/6J strain, associated with higher abundance of a putative short transcript form.
To estimate the frequency of alternative termination, a subset of 5,145 contigs was properly aligned in their 5' to 3' orientation.
The sequence variation in the G gene was due to nucleotide substitutions, in-frame insertions, and the use of alternative termination transcription codons.
In both cases, a specific transcript is likely to be obtained from the initiation or termination of transcription downstream of alternative promoters and polyadenylation sites, respectively.
The percentage of genes with evidence of alternative termination ranged from 27% in the RL-SAGE mycelium library to 35% in the MPSS mycelium library.
This mechanism of alternative transcription termination could define the length of 3′ extensions in fry1 and xrn3 mutant backgrounds.
The work of the FANTOM and ENCODE projects has revealed the true complexity of the mammalian transcriptome, highlighting the impact of alternative initiation, termination and splicing on the proteome, and the prevalence of multiple different classes of non-coding RNAs (ncRNAs) [ 8- 11].
To show a new technique of hysterotomy via laparoscopy for a failed termination of pregnancy as an alternative for a hysterotomy via laparotomy.
