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Sanger sequencing was performed using the Big Dye termination kit.
In all, 100 colonies were randomly picked and sequenced using the PRISM dye termination kit™ (ABI, Chiba, Japan).
Automated sequences were generated on an ABI PRISM 310 Genetic Analyzer according to Sanger et al. [ 99] with a BigDye Termination kit (Applied Biosystems, USA).
Amplicons have been directly sequenced using BigDye 3.1 Sequence Termination kit in ABI3100 Genetic Analyzer (Applied Bio Systems, Foster City, CA, USA).
Both strands were directly sequenced using a commercially available Cy™ Dye Termination Kit using the MegaBACE 1000 automated DNA sequencer (GE Healthcare).
One μL of this product was sequenced with the same primers in a 5 μL reaction using the ABI Big Dye dideoxynucelotide termination kit (Applied Biosystem, Foster City, California).
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The plasmids were end-sequenced using BigDye Termination kits on Applied Biosystems 3730xl DNA Analysers.
The plasmids were end-sequenced by the Genoscope using BigDye Termination kits on Applied Biosystems 3730xl DNA Analysers.
The purified cDNA was sequenced by using the ABI PRISM Dye Terminator kit version 3.1 (Applied Biosystems, Foster City, CA, USA), which uses the chain termination methods with dideoxyribonucleotides labeled with different fluorophores for each nucleotide on the 3′ end (17 ).
DNA sequencing was performed with the ABI-PRISM Dye Termination Sequencing kit and ABI 373-A genetic analyzer (Applied Biosystems, Carlsbad, CA, USA).
DNA sequencing was performed with the ABI-PRISM Dye Termination Sequencing kit and an ABI 373-A genetic analyzer (Applied Biosystems, Carlsbad, CA, USA).
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