In [ psi - ] cells, the Sup35 protein acts as a translation termination factor that, together with Sup45, recognizes stop codons and terminates translation.
The third is TTF1, a transcription termination factor that regulates ribosomal gene transcription (60).
Transcription termination factor Rho is an ATP-dependent ring-shaped molecular motor that tracks along RNA to dissociate RNA DNA duplexes and transcription complexes in its path.
[URE3] is a prion of Ure2p, a regulator of nitrogen catabolism, while [PSI +] is a prion of Sup35p, a subunit of the translation termination factor Sup35p.
Transcription termination factor Rho forms ring-shaped hexameric structures that load onto segments of the nascent RNA transcript that are C-rich and mostly single-stranded.
Termination, but not antitermination, was restored by the bacterial termination factor NusA. Surprisingly, a mutant that lacks the entire zinc binding domain regained a partial response to put.
Recent crystal structures of human mitochondrial termination factor MTERF1 and Bacillus cereus alkylpurine DNA glycosylase AlkD bound to DNA revealed two new superhelical tandem repeat architectures capable of wrapping around the double helix in unique ways.
In this study, the suppressor mutation was identified to be a point mutation in rho encoding the transcription termination factor Rho. Strain RE2 still showed retarded growth despite the mutation rho696.
Depletion of the translation termination factor Sup35p into [PSI+] prion aggregates reduces efficiency of termination, which, in combination with the SUQ5 tRNA, suppresses ade2 1.
We have investigated the role of Sse1 in the de novo formation and propagation of [PSI+], the prion form of the translation termination factor, Sup35.
Sup35 is a translation termination factor and conversion to the [PSI+] prion state inactivates Sup35 molecules thereby increasing the levels of nonsense suppression [1], [6], [7].
