Sentence examples for termination elements from inspiring English sources

This widely used reporter gene's coding region is unlikely to contain functioning termination elements, and furthermore, also unlikely to contain functioning promoter elements that could contribute to background transcription.

In this regard, we sometimes co-expressed two or three genes within one plasmid by placing the genes flanked by the above promoters and inserting transcription termination elements between them, because the selectable marker genes available are limited (URA3, HIS3, TRP1, and LEU2).

However, in this study, any arrangement of two transgenes was more susceptible to silencing than a single transgene and silencing occurred despite the presence of the SV40 polyadenylation/transcription termination elements downstream of the transgenes.

Separating the virion- and complementary-sense open reading frames (ORFs) are the long intergenic region (LIR) - comprising transcriptional promoter elements and the virion strand origin of replication (v-ori) [ 30] - and a short intergenic region (SIR), where the transcription termination elements and the complementary strand origin of replication reside.

To restrict virus-mediated transduction to glutamatergic hippocampal neurons our approach was to excise a transcriptional termination element preceding the cDNA in the AAV-expression cassette by providing Cre-recombinase in trans (Fig. 1A).

Nevertheless, potential problems of UCOE-mediated host promoter activation/inhibition can be avoided by placement of a transcriptional termination element 5′ of the A2UCOE.

A well-studied anti-termination element is the so-called T-box.

Although much is known about the role of pre-mRNA processing in termination, our understanding of the characteristics and generality of terminator elements is limited.

These data provide further compelling evidence that Pol II termination on the CCNB1 gene is mediated by the CoTC termination mechanism and confirm that, using the CLIP-seq strategy, we have successfully identified authentic Pol II terminator elements.

Each terminator element promotes efficient Pol II termination with the most potent, from the CCNB1 and PTCH2 gene 3′ flanking regions, mediating 100% Pol II termination in nuclear run on assays.

This terminator element was placed downstream from the transgene termination codon.