Sentence examples for termination cycle from inspiring English sources

The cDNA was sent for semi-nested PCR with primers used in a previous report [ 27], the PCR product was purified, and auto-sequencing was performed with the forward primer on a 3730 ABI automatic sequencer with BigDye Terminator v3.1 Termination Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Perkin-Elmer, Foster City, CA).

The DNA was reconstituted in 30 µl TVLE (tris very low ethylenediaminetetraacetic acid [EDTA], 10 mM Tris/0.05 mM EDTA) and 4 µl was used for chain termination cycle sequencing using BigDye Terminator v3.1.

Plasmid DNA was directly subjected to sequencing in CEQ 8000 Genetic Analyzer (Beckmann Coulter, Brea, CA, USA) using the Dye Termination Cycle Sequencing Kit (Beckmann Coulter).

DNA sequencing kit (ABI PRISM 310 XL with dye termination cycle sequencing ready reaction kit) was obtained from Perkin Elmer, (Norwalk, CT).

Sequencing was performed with the reagents of the ABI Prism BigDye termination cycle sequencing kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.

Purified PCR products were sequenced using the Big-Dye Termination cycle sequencing reactions and an ABI Prism 3100xl sequencer (Applied Biosystems, Inc., Foster city, CA).

In perennial tomato, the orthologue SFT has been shown to regulate diverse growth processes, such as the flowering, growth and termination cycles typical of perennial plants, leaf maturation, growth of stems, and the formation of abscission zones [ 48].

Partial sequences of cloned Bm86 orthologs were obtained by double-stranded dye-termination cycle sequencing (Core Sequencing Facility, Department of Biochemistry and Molecular Biology, Noble Research Center, Oklahoma State University and Secugen S.L, Madrid, Spain).

Amplified 16S rDNA, groESL and gltA fragments were resin purified (Wizard, Promega) and cloned into pGEM-T vector (Promega) for sequencing both strands by double-stranded dye-termination cycle sequencing (Core Sequencing Facility, Department of Biochemistry and Molecular Biology, Noble Research Center, Oklahoma State University).

Amplified Anaplasma 16S rDNA fragments were resin purified (Wizard, Promega) and cloned into pGEM-T vector (Promega) for sequencing both strands by double-stranded dye-termination cycle sequencing (Core Sequencing Facility, Department of Biochemistry and Molecular Biology, Noble Research Center, Oklahoma State University).

Nucleotide sequences of cDNA clones were determined by the dideoxynucleotide chain termination method, using BigDye Terminator cycle sequencing chemistry (Applied Biosystems) in an ABI PRISM 377 DNA sequencer.