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Upon termination, cells were scratched and lysed with 1 mL of TRIzol Reagent (Life Technologies) and stored at −80°C until RNA isolation, as described in earlier sections.
At termination, cells are rinsed, fixed with 10% neutral-buffered formalin for 10 min, and stored in 70% ethanol until processed for either histochemical localization of localization of senescence, or immunohistochemical detection of senescence as described above.
At termination, cells in monolayer were trypsinized, centrifuged at room temperature for 5 minutes at 500 rpm and resuspended in 500 μl lysis buffer (58.44 mg/ml NaCl) (Sigma); 0.1 mg/ml trypsin inhibitor (Sigma); 0.1% (v/v) triton X-100 (Sigma).
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The insignificant increase in MFI may be ascribed to the fact that at the time of experimental termination, the cells may have already surpassed the early apoptotic stage.
The statistically insignificant increase may be due to the fact that PS externalization is an early sign of apoptosis and that, at the time of experiment termination, the cells may have already surpassed the early apoptotic stage.
At culture termination, the cells were removed from the bone slices, resorption events were visualized with toluidine blue (Sigma-Aldrich), and analyzed by light microscopy for the percentage of resorbed surface and resorption depths using a 100-point grid as explained in Boissy et al. and Soe and Delaisse.
Secondary walls develop in some cell types after the termination of cell expansion, and these walls usually contain lignin in addition to polysaccharides.
The active species of both enzyme/prodrug systems are nucleotide analogues that interfere with DNA synthesis causing chain termination and cell death exclusively in proliferating cells.
Our observations suggest that oil quality improved during cell division; and subsequently plummeted following the termination of cell division (initiation of fatty acid accumulation).
To investigate the molecular mechanisms through which Tim regulates replication termination and cell cycle progression, we generated a stable human cell line expressing FLAG-tagged Tim protein.
Consistent with a role in the termination of cell proliferation, dap expression in the larval OL has been tightly correlated with cells ending proliferation [36].
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