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Several specialized C-terminal domains involved in chain termination and release of the final peptide product have also been identified [ 16, 17].
Furthermore, theioesterase (TE) domains and reduction domains are encoded in the final module for chain termination and release of the polypeptide or polyketide natural product.
We have reworded the concluding remarks of the Abstract and the Discussion, in order to reflect the comments of the reviewer concerning the plausibility of the proposed mechanism of primer termination and release by Pol α.
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Having reconstituted the activities of the Bref-PKS, we then investigated the role of Bref-TH in chain termination and product release.
The ribosome then moves along the mRNA joining together amino acids brought to it by transfer RNA (tRNA)—until it reaches a termination site and releases the protein.
Stop codons are recognized by the translation apparatus; normally this event leads to termination of translation and release of the encoded protein.
Also, while termination of elongation and release of free FAs appears to occur in both seed tissues as well as in some of the vegetative tissues, DGAT expression in vegetative tissues is too low to detect with the EST counts.
After termination of synthesis and release of the completed primer, the physical tethering of Pol α to primase would facilitate its recycling onto the 3′-end of the next RNA primer (Nunez-Ramirez et al., 2011; Kilkenny et al., 2012).
In this study, of over 100 unidentified Pol II transcribed genes, it was found that Pol II termination and pre-mRNA release occurred prior to pre-mRNA 3′ end processing for 64% of these genes (Osheim et al., 2002).
After translocation through the NPC, the Ci-155-importin α3/β complex encounters RanGTP, leading to cargo association and release for termination of the transport cycle.
These malonyl CoA analogs, when added to fermentation broths, underwent chain elongation, β-processing, and chain termination to release off-loaded intermediates.
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