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Thermal decomposition analysis demonstrated that the improvement of flame retardancy of treated wood was achieved through dehydration of polysaccharides and forming of protective char shield, terminating heat and oxygen transferring.
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Hormonal treatments were terminated by heat shock (5 min, 100 °C).
The reaction was terminated by heat inactivation.
After the purified PrC was incubated with the nematode cuticle (1 mg/ml) for 20 min, the reaction was terminated by heat at 100°C for 5 min. The reaction mixture was collected and applied to a centrifugal iCONTM concentrator (MWCO 3 kD, Pierce, Rockford, IL).
The reaction was terminated by heat inactivation (70 °C, 15 min).
The reaction was terminated by heat inactivation at 65 °C for 10 min.
The reaction was terminating by heating for 20 min at 65°C.
The reaction was terminated by heat denaturation at 95 °C for 5 min, in a DNA engine thermocycler (BioRad, Hercules, CA).
Reactions were terminated by heat inactivation at 90°C and RNA/DNA recovered by isopropanol precipitation with 1 μl GlycoBlue (Ambion) as a coprecipitant.
Reactions were performed by adding the protein substrate to a solution containing PCAF (20 nM, 100 μM AcCoA, 50 mM Tris, pH 8.0, 0.1 mM EDTA), allowing the reaction to proceed for 10 min at 30 °C, and terminating by heating to 100 °C.
The reaction was incubated at 42°C for 1 h and terminated by heat inactivation at 95°C for 5 min. For primer design the ITGB6 coding sequence from 5 species (Bos taurus (Genbank: NM_174698), Homo sapiens (Genbank: NM_000888), Mus musculus (Genbank: NM_021359) and Rattus norwegicus (Genbank: NM_001004263)) were aligned using "Multialin" [ 27].
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