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The reaction was terminating by heating for 20 min at 65°C.
Reactions were performed by adding the protein substrate to a solution containing PCAF (20 nM, 100 μM AcCoA, 50 mM Tris, pH 8.0, 0.1 mM EDTA), allowing the reaction to proceed for 10 min at 30 °C, and terminating by heating to 100 °C.
Reactions were started by the addition of 5 μl Mg/ATP mix (15 mℳ MgCl2 and 100 μℳ ATP, with 18.5 kBq γ-P-ATP per well) and incubated at 30 °C for 30 min before terminating by the addition of 25 μl 75 mℳ H3PO4.
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The neurotransmitter is terminated by uptake into the presynaptic terminal.
It purportedly has been terminated by Bain, though 3Com has disputed this termination.
The pregnancy is terminated by the mother.
Pregnancy can be terminated by chemical means.
Socrates' life was terminated by his trial and execution.
"It can only be terminated by mutual consent.
This harsh viceregal power was terminated by Austrian conquest (1707).
He was terminated by the Los Angeles Board of Education in February.
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