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Transformation of chlorite to ankerite is the dominant reaction occluding the shale porosity in most simulations: from 4000 to 7500 a, this carbonation seals the reservoir and terminates reaction.
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The container was then turned into ice bath to terminate reaction.
When performing Fe VI -US samples taken should be added to a small amount oFe VI -US·L−1 samplestakenulfate to terminate reaction, with the shoulds being addedzed aftor centrifugation.
The terminated reaction was spotted onto a SAM2 Biotin Capture membrane (Promega) and further treated according to the instructions of the manufacturer.
Furthermore, three-body chain terminating reactions involving H2O are enhanced.
The terminated reactions were processed by conventional SDS-PAGE under denaturing and reducing conditions and transferred to nitrocellulose membranes as described [9].
In all experiments the terminated reactions were analysed on native 20% polyacrylamide gels containing 1× TBE.
Azo reagent NO.1, Azo reagent NO.2 and Azo reagent NO.3 were added to the reaction system successively to terminate reactions.
Separation and quantification of UDP-sugars in terminated reactions was performed by HPAEC analysis using a Dionex Ultimate 3000 system (Thermo Fisher) with detection at 262 nm.
Sulfuric acid (1 N) was added to terminate reactions, and the absorbance at 450 nm was determined by a microtiter plate reader (TECAN, Grödig, Austria).
The terminated reactions were spotted onto a SAM2 Biotin Capture membrane (Promega) and further processed according to the manufacturer's instructions.
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