Hence, the reaction is expected to both terminate (producing sticky ends essential for the splicing phase) and run through the Ara-C (producing blunt ends; this feature is essential to the second PCR reaction).
Another example of transcriptional silencing initiated in response to processing defects was reported for convergent genes in S. pombe, where failure to terminate can produce overlapping read-through transcripts [ 101].
His election to office, Henry's rant continued, "would terminate in producing rivulets of blood throughout the land".
To detect nascent RNAs associated with paused TECs (pause RNAs) and full-length terminated transcripts produced from this template, we isolated total RNA and used Northern blotting with a locked-nucleic-acid (LNA) probe, as described previously (Deighan et al., 2011).
Furthermore, it appears that the B-class genes themselves have likely been the loci of the masculinizing mutations that terminate potentially hermaphroditic flowers before they can produce carpels.
A copolymer consisting of the combination of the LP-33 polysulfide and hydroxyl terminated polybutadiene is produced as a candidate low ignition temperature ramjet fuel.
We conclude that translation of EBNAα is terminated prematurely to produce truncated polypeptides which fit the definition of defective ribosomal products or DRiPs as originally proposed by Yewdell and colleagues [17].
This reaction mainly consists of the chain scission under ultrasonic irradiation, the end chain reaction of the produced macroradicals with MAH, and the terminated reaction of the produced succinyl radicals with the macroradicals or H* radicals through recombination or dismutation.
Because terminating ribosomes produce a characteristic peak of ribosome density over annotated stop codons, we asked whether the stop codons that terminate the C-terminal extensions also showed this behavior.
It lasted for 13 years and was terminated in 1986 after producing 688 nurse practitioners, due to a reduction in qualified applicants.
