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At low infectious doses (102 PFU or less) mice survived up to 21 days, at which point the experiment was terminated (data not shown).
Disease progress as determined by AUDPC was significantly (P < 0.05) greater in Bd3-1 (31.92) than in Bd21 (20.16) (Additional file 3), although there was no significant difference in conidial production at 13 dpi, when the experiment was terminated (data not shown).
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In addition to the length of the direct repeats, analysis of the 15 integration site sequences showed that all viral sequences, with the exception of the left LTR end of the proviral clone C-3, were terminated with the conserved CA dinucleotide at the 3' end (data not shown), indicating that the viral DNA ends were correctly cleaved by IN [12].
We are able to generate tails with a tight distribution around an optimal length of 30 bps by including a low concentration of a terminating dideoxycytidine nucleotide (data not shown).
At the 13th week when most other schemes terminate, the completion rate was 58% (data not shown).
More recently, a separate NDM-1 Enterobacter cloacae outbreak was observed in the hospital, albeit in association with a different plasmid vector (data not shown), and was terminated following fumigation of a number of the clinical units, demonstrating the need for ongoing surveillance and comprehensive infection control.
Indeed, there is no electron density for the flexible thioether-containing chain that terminates in the important free primary amine (data not shown).
Due to the development of non-specific T cell/tumor alloreactivity and severe GVHD (data not shown), the experiment was terminated on day 84 post-tumor inoculation.
These spontaneous bursts appeared as all-or-none voltage-dependent events that could be initiated by depolarizing current pulses (data not shown) and forced to terminate prematurely by negative current pulses.
We have also observed this effect with microarrays synthesized with oligonucleotides of increasing lengths terminated by a coupling with a Cy3 or Cy5 phosphoramidite (data not shown).
We found that 5/6 Anp32e−/− mice were still alive at 80 weeks of age when the experiment was terminated; this proportion was similar to that in the Anp32e+/− group (data not shown).
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