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Each blot was sealed in a heat-sealable bag with the dye solution; stored at room temperature in the dark for typically 50 min to 2 h; and then incubated in 20 mM Tris, pH 7.5, and 1 mM EDTA for 5 min at room temperature to terminate the development.
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Poor pharmacokinetic properties and toxicity of the compounds are one of the main reasons for terminating the development of drug candidates [ 40].
In vitro, TDIF could independently suppress the differentiation of procambial cells to treacheary elements and promote cell division, but is unable to terminate the RAM development and cause root development retardation [ 42].
The sections were placed in distilled water to terminate the DAB development reaction and mounted with 25% ethanol in PBS on to positively charged glass slides (ThermoFisher).
In some cases, the subject will be terminated before the development of the relevant cognitive capacities, thus side-stepping the entire problem.
The test was terminated following the development of this fracture due to the considerable drop in the load-carrying capacity. Figure 8 illustrates the crack patterns in the end columns at the end of the test.
As a result the trial was terminated and the development of arzoxifene for established breast cancer was discontinued.
In order to determine the most sensitive stage of ET signaling that induced intestinal phenotype in the present model, we took advantage of the shell-less culture to administer phosphoramidon to the embryo at different development stages and terminate the treatment by washing out the medium (Table 2).
tries to terminate the deal.
They'll terminate the contract.
They immediately terminate the relationship.
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