Sentence examples for terminate reaction from inspiring English sources
The container was then turned into ice bath to terminate reaction.
Take 0.5g plant root samples treated by different concentrations of copper; immerse in 10 mL beaker with the mixed liquid of 0.4% TTC and 66 mmol/L phosphate buffer solution (pH = 7.0), keep at 37°C for 3 h, then add 1 mol/L of sulfuric acid 2 mL to terminate reaction.
In details, after being treated with folic acid-DNA tetra-Dox for a period of time at 100 μM, cells were digested by 0.25% trypsin for 30 s, and then, the equal volume of complete medium was added to terminate reaction.
When performing Fe VI -US samples taken should be added to a small amount oFe VI -US·L−1 samplestakenulfate to terminate reaction, with the shoulds being addedzed aftor centrifugation.
AChE activity was determined by AChE kits (Nanjing Institute of Biological Engineering, China), operating according to its instruction, as follows: 50 μL substrate solution and 50 μL chromogenic liquid were added to 3 μL samples, incubated 6 min at 37°C after blending, and then 3 μL stop solution was added quickly to terminate reaction.
Azo reagent NO.1, Azo reagent NO.2 and Azo reagent NO.3 were added to the reaction system successively to terminate reactions.
Sulfuric acid (1 N) was added to terminate reactions, and the absorbance at 450 nm was determined by a microtiter plate reader (TECAN, Grödig, Austria).
The terminated reaction was spotted onto a SAM2 Biotin Capture membrane (Promega) and further treated according to the instructions of the manufacturer.
The terminated reactions were processed by conventional SDS-PAGE under denaturing and reducing conditions and transferred to nitrocellulose membranes as described [9].
In all experiments the terminated reactions were analysed on native 20% polyacrylamide gels containing 1× TBE.
Separation and quantification of UDP-sugars in terminated reactions was performed by HPAEC analysis using a Dionex Ultimate 3000 system (Thermo Fisher) with detection at 262 nm.
