As a positive control, trypsin-treatment changed the electrophoretic mobility of Calnexin by removing the 90 cytosolic C-terminal residues of this type I protein (Fig. 1D, lane 6, Calnexin ΔC).
Because bar-1 loss-of-function mutants do not have seam cell terminal differentiation defects of this type (J.
Altogether > 300 terminals of each type were analysed in three sections per animal, and in three animals per genotype.
The ClpX family of proteins contains a C4 zinc-binding domain in the N-terminal region and this type of domain has been implicated in nucleic acid binding in both eukaryotic and prokaryotic organisms [43,44].
The use of this type of terminal water filter is an appropriate method to protect immunocompromised patients from water-borne pathogens such as Legionella.
Interestingly, proteins remarkably similar to the C-terminal half of this CPRas type including the potential zinc finger but lacking even vestiges of an N-terminal cpRAS domain can be found in several taxa, namely Dictyostelium, Entamoeba dispar, some metazoans (the mollusc Lottia gigantea, the annelid Capitella sp. I, Strongylocentrotus), and ciliates (Fig. 2B).
In any case, the reporting of QMET- (found also in this work), QMEN- and QMDT- N terminal peptides indicates that this type of processing may occur in LMW-GS.
The intensity of MGL-immunostaining was highly variable even between terminals of the same type.
gambiae LCR substantially exceed the 1 2 kb maximum length reported for any other foldback elements, and lack the terminal blocks of subrepeats often associated with this type of TE.
They are found in a variety of cytosolic positions, including loops, at the C- and N- terminal end of type I and II membrane proteins respectively [ 25].
For the urinary evaluation of degradation products of C-terminal telopeptides of Type I human collagen the Urine CrossLaps® ELISA Kit has been employed and for products of C-terminal telopeptides of type II the Urine CartiLaps® ELISA kit (both from Nordic Bioscience Diagnostic DK, Italian subsidiary Pantec Turin) has been employed.
