In particular, the introduction of a truncated N-terminal version of this protein resulted in baseline expression of the LevQRST targets in the presence of inducing substrates, but higher levels of expression of these genes in the presence of carbohydrates that do not normally induce expression.
For the co-purification of interaction partners with NirF, the STrEP-tagged (C-terminal) version of NirF was produced in the P. aeruginosa PAO1 strain RM301 lacking the native NirF.
In order to experimentally verify the bioinformatic prediction that NirF from P. aeruginosa is a membrane attached lipoprotein, we produced the STrEP-tagged (C-terminal) version of NirF in P. aeruginosa PAO1 RM301 lacking the native NirF as described above for the affinity co-purification experiments.
To test this, we performed chromatin immunoprecipitation (ChIP) assays with MOVAS cells transiently transfected with a plasmid encoding a FLAG-tagged (N-terminal) version of HOXC11 (F-HOXC11); endogenous Hoxc11 expression was not detected in MOVAS cells by semi-quantitative PCR (supplementary material Fig. S3).
Similar results were obtained whenever an N-terminal or a C-terminal tagged version of the proteins was used.
The telomere capping role of POT1B was inferred from an experiment in which a C-terminal truncated version of the POT1B protein harboring only the N-terminal OB domains was expressed in wild-type plants [77].
A role for the N-terminal 15 amino acids in the DNA-binding property of MsDps2 has been indicated by AFM analysis of an N-terminal deleted version of the protein.
A C-terminal truncated version of the protein (991 aa) was found to be encoded by a short form of the vegfr2gene [ 3].
An N-terminal truncated version of Lsk1 rescues the thermosensitive defect of lsk1 deletion and shows in vitro kinase activity similar to the wt, which suggests a regulatory rather than catalytic role for the N-terminal extension.
The three-dimensional (3D) structure of reconstituted N-terminal truncated version of human Top1 in complex with a 22 bp DNA molecule shows the enzyme organized in multiple domains, which 'clamp on' the DNA molecule [ 3].
For protein expression in yeast, the N-terminal truncated version of SsLPPS was sub-cloned into the first multiple cloning site of the expression vector pESC-Leu (Stratagene, http://www.genomics.agilent.com), resulting in pESC-Leu: SsLPPS.
