In the case of metabolism, full viability is restored only by mutations in the 5 successive terminal reactions of the three-carbon part of glycolysis/gluconeogenesis out of 21 mutations leading to inactivity or absence of enzymes of the central carbon metabolism.
Protein-protein interactions between terminal docking domains of successive multienzymes promote their correct positioning within the assembly line, but because the overall complex is not stable in vitro, the key interactions have not been identified.
The PAMAM dendrimers with ammonia as the core molecule possess an ester group (R-COO-R) and amine (R-CO-NH2) group as terminal surfaces in the successive half and full generation.
STD is thought to be due to a depletion of the readily releasable pool of synaptic vesicles, while STF is thought to be the result of Ca2+ build up in the terminal due to rapid successive depolarizations (Desai-Shah and Cooper, 2009).
Successive C-terminal truncation of mRFP1 showed that fluorescence was abolished after 10 amino acids were removed (Fig. S4, S5).
Successive N-terminal shortening of ICL3 (constructs #1- 5) progressively reduced cell surface expression, ligand-induced IP formation and luciferase activity but atropine failed to modulate luciferase activity.
The enzyme's movement likely arose from its well-characterized processive mode of cellulose-chain degradation, involving successive cleavages of terminal cellobiose units from the same chain without intermediate chain release from enzyme6.
Assuming that the redox reactions proceed under kinetic constraints due to microbial activities rather than thermodynamic equilibrium, the successive use of terminal electron acceptors can be rationalized in terms of the redox reaction energy yields [24].
SM-POD [25]: task offloading is based on a series of successive classifications considering mobile terminal capacities, task characteristics, or communication channel state.
The sample was digested with trypsin and the mixture was subjected to successive cycles of N-terminal degradation.
In traditional protein chemistry, proteins were identified by de novo sequencing using automated Edman degradation which is based on successive removal of N-terminal amino acids by chemical methods [ 6].
